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•E. coli strains•

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software, database
cloning, protein expression
biophysics, crystallisation
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PSF E. coli
expression

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ORFer software
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We use E. coli SCS1 (Stratagene) for high-throughput cloning and protein expression. Transformation competent cells already carry the pSE111 plasmid, that provides for overexpression of lac repressor and a rare tRNA. For labeling of proteins with seleno-methionine, the met-auxotroph strain B834 is used.

Strains E. coli Purpose Genotype
SCS1 K12 High throughput cloning, protein expression recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1
BL21 B Protein expression E. coli B F- ompT hsdS6(rB- mB-) gal dcm
B834 B Protein expression, Se-Met labeling F- ompT hsdS6(rB- mB-) gal dcm met

 

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© 2003 by V. Sievert, Konrad Büssow last changed 12 Sep 2006