PSF
•Project structure•
Abstract
The
term "Protein Structure Factory" stands for a common initiative
of structural biologists from the Berlin area and the German Human Genome
Project (DHGP). The Protein Structure Factory represents an integrative
approach to the high-throughput analysis of three-dimensional protein
structures.
We have set out to establish an analytical infrastructure to characterize
human proteins whose cDNA sequences are available at the Resource
Center of the DHGP in Berlin. Target
proteins are selected on grounds of predicted novelty of the structure
as well as medical or biological relevance. We combine sequence analysis
with bioinformatics, automated gene expression and protein purification,
determination of a biophysical fingerprint and finally the three-dimensional
protein structure analysis by either NMR spectroscopy or X-ray diffraction
techniques. The crystal structure analysis will use the synchrotron radiation
of the storage ring BESSY in Berlin.
Detailed summaries on the sub-projects from the orginal grant proposal are available.
The project pipeline consists of these main components:
- Target selection, cloning, protein expression and purification
- NMR
- Biophysical characterisation and protein crystallisation
- X-ray diffraction analysis
- database,
management
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© 2003 PSF, last changed 23 Nov 2009