•Biophysics and Crystallisation•

Biophysical characterisation and protein crystallisation are closely connected at the PSF.

Protein samples are characterised by the following methods before crystallisation:

• UV-spectroscopy
  • Concentration
• SDS-PAGE
  • Homogeneity
• Dynamic light scattering
  • Particle size
  • Monodisperse/aggregated
• Fourier-transform infrared spectroscopy (FT-IR)
  • Protein folded/unfolded (random coil)
  • Secondary structure elements
• Micro-calorimetry
  • Stability in different buffer conditions
• Tryptophan fluorescence spectroscopy
  • Effect of buffer conditions on protein conformation
• [Example - Concentration Test] (personal pages Ulf Lenski)
• [Example - Biophysical Data] (personal pages Ulf Lenski)
• [Example - Observation] (personal pages Ulf Lenski)

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© 2003 PSF, last changed 28 Mar 2007