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•Cloning and protein expression•

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overview
software, database
cloning, protein expression
biophysics, crystallisation
NMR
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Expression in E. coli

The Protein Structure Factory studies the structures of human proteins. Target lists are compiled in collaboration with the group of Peer Bork at the EMBL Heidelberg. The German Resource Center provides the cDNA starting material. cDNA fragments are cloned into yeast and E. coli expression vectors. High throughput methods for cloning and characterisation have been established.

E. coli fermentation and large scale purification are carried out by Alpha Bioverfahrenstechnik.

Please check our new project PSPF (Protein Sample Production Facility.

Experimental details, vectors, strains are available on the E. coli cloning and protein expression page.

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© 2003 PSF, last changed 11 Jul 2007